布鲁菌16M感染后宿主免疫相关蛋白质泛素化修饰的差异分析

doi:10.11843/j.issn.0366-6964.2019.11.013

Abstract

Abstract: This experiment was conducted to investigate the expression level of ubiquitinated modified proteins in host cells infected with Brucella in early stage and screen out the key regulatory proteins that affect the process of immune response. By using Label-free and ubiquitination enrichment technology and high resolution LC-MS/MS combined quantitative proteomics research strategy, ubiquitination proteomics quantitative studies were carried out on Brucella 16M infected macrophages and uninfected macrophages after 11 hours (5 hours' infection, 6 hours' intracellular replication). Database retrieval analysis was performed on the proteins which were corresponding to the ubiquitination sites that differentially expressed by 16M infected macrophages and uninfected macrophages. The key proteins that can cause host immunosuppression after 16M infection of macrophages were screened out by bioinformatics method. In this study, 580 ubiquitination sites were identified on 349 proteins. The ubiquitination level of 259 sites on 167 proteins in the 16M infection group was up-regulated compared with the uninfected group, 321 sites on 212 proteins were down-regulated (difference multiple >1.5, P<0.05); There were 35 ubiquitination-modified differentially expressed proteins may be related to the host immune response after Brucella infection. Among them, we found 27 ubiquitinated down-regulated proteins such as Bcap31, Btk, Faf1 and Akap31; one up-regulated ubiquitinated protein, Ubqln1, may be the key protein causing immunosuppression after Brucella infection. In this study, ubiquitinated modified proteins differentially expressed in host cell immune response to Brucella 16M infection were screened and obtained. It was preliminarily revealed that Brucella can affect the ubiquitinated modification of related proteins in host immune signaling pathway, autophagy and apoptosis, so as to further study the regulation of perennial status after Brucella infection. The main ubiquitination modification to complete the molecular mechanism of immune escape provides a theoretical basis.

CLC Number:

S852.614

ZHOU Yucheng, GUO Mengnan, CHENG Shipeng, ZHANG Haiwei, ZHOU Manli, QIAO Lianjiang, YANG Yanling. Differential Analysis of Ubiquitination Modification of Host Immune-related Proteins after Brucella 16M Infection[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(11): 2290-2301.

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Differential Analysis of Ubiquitination Modification of Host Immune-related Proteins after Brucella 16M Infection

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